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. 2018 Mar 1;9:70. doi: 10.3389/fphar.2018.00070

Figure 5.

Figure 5

Glucoevatromonoside impairs cell growth and induces caspase-dependent apoptosis in U937. (A) GEV reduces proliferation of U937 cells in a concentration-dependent manner; (B) Analysis of cell death performed by trypan blue staining after 24 and 48 h of treatment at 10, 50, and 100 nM; (C) GEV induces apoptotic nuclear morphology. Arrows show fragmented nuclei; (D) Quantification of cell death by Hoechst staining of fragmented nuclei in treated cells vs. untreated cells; (E) Effect of zVAD on cell death induction by GEV at 50 and 100 nM evaluated by Hoechst staining; (F) U937 cells were treated with GEV, extracted proteins were separated by SDS-PAGE and analyzed by Western blot against caspase-3. Etoposide was used as positive control at 50 μM for 3 h. Data represent the mean ± SD of three independent experiments (ANOVA followed by Dunnett's test). Significance is reported as: **P < 0.01, ***P < 0.001, ****P < 0.0001 compared to controls.