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. 2018 Mar 5;19:178. doi: 10.1186/s12864-018-4545-9

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Phylogenetic distribution of Triticeae and Brachypodium MKKs. PK domains of wheat, barley, rye and triticale MKKs were aligned with BdMKKs, and the tree rooted with HsMEK1. Descriptive gene names are followed by chromosome number when available. Accessions used in the analysis are marked in bold in Additional file 3. BdMKKs are bolded in tree to visually separate them from the sequences identified herein. Only accessions with complete PK domains are included in the analysis. MKKs are divided into four clades (a, b, c, and d). MEME analysis shows amino acid distribution of the NTF-2 domain conserved in the C-terminal extensions of MKK3s. An additional tree is presented for clade D (MKK10s), where the Triticeae species were aligned with Brachypodium, rice (Os) and maize (Zm) and the tree rooted with HsMEK1. Font colours are used to distinguish MKK10 orthologues from different species: MKK10–1 (red), MKK10–2 (orange), MKK10–3 (blue), MKK10–4 (purple), MKK10–5 (teal)