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. 2018 Mar 5;19:178. doi: 10.1186/s12864-018-4545-9

Fig. 7.

Fig. 7

Tissue-specific relative expression of wheat MPKs at different developmental stages. The relative expression was measured through qRT-PCR in different tissues at indicated time points of development. Normalized relative expression was calculated from the cycle threshold values using qBASE Plus software, where the calculated expression level is relative to the lowest value in the data set. Bars represent standard error of means (n = 3). Statistical analysis was carried out in SPSS 15.0 using a mixed linear model with an REML estimation method (gene = fixed effect). Bonferroni multiple comparison adjustments were employed to analyze the main effects (p ≤ 0.05)