Fig. 3.

a Quantitative RT-PCR (qRT-PCR) showed a twofold decrease of PTCH1 expression levels in the germline and tumor tissue in comparison to the peripheral blood-derived cDNA from the parents and b a tenfold significant increased GLI1 expression level in the tumor sample and a sixfold lower GLI1 expression in the parents compared to the patients’ germline. c The PTCH2 expression level in the tumor sample was threefold increased in comparison to the germline of the patient and his parents. d SMO was 500-fold increased in the tumor tissue, whereas all three germline samples showed low expression levels