Figure 8.
Validation by qRT-PCR of microarray data, corresponding to genes involved in primary metabolism, secondary metabolism, and differentiation. (A) Validation of genes overexpressed in ΔargR mutant. (B) Validation of genes underexpressed in ΔargR mutant. The expression is presented in relation to the control strain, taken as 1. Upper panel indicate gene name and culture time at which qRT-PCR was performed. Corresponding lower panel shows gene profile of the microarray study. Black bars and black lines correspond to control strain S. coelicolor M145. Gray bars and gray lanes correspond to S. coelicolor ΔargR. (C) qPCR vs. microarray: Scatter plot correlating to transcriptomic changes as measured by microarray analysis and qRT-PCR of all genes validated. R2 was 0.926.