Inflammatory gene induction and COX-2 protein by LTD4 and PGE2 in LAD2 cells. LAD2 cells were stimulated with 0.5 μmol/L LTD4, PGE2, or both; transcript levels of MIP-1β, TNF-α, IL-8, COX-2, and COX-1 were analyzed by means of real-time PCR at 2 hours of stimulation (A–E); and COX-2 protein levels were determined (F) in cell lysates. *P < .05, **P < .01, and ***P < .001.