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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: J Mol Cell Cardiol. 2018 Jan 4;115:104–114. doi: 10.1016/j.yjmcc.2018.01.003

Figure 4. PKC activation mediates a biphasic rearrangement of the actin cytoskeleton.

Figure 4

A, Western blot of G- and F-actin and summary data of the ratio of G-actin vs. F-actin in left ventricular myocardium after TAB in mice. PKC inhibitor enzastaurin (Enz) abolished actin depolymerization. n=3 hearts for each time point. * p<0.05 vs 0 d TAB+DMSO group; # p<0.05 vs 2d TAB+DMSO group. B, Western blot of G- and F-actin and summary data of the ratio of G-actin vs. F-actin in cultured cardiomyocytes 30 min or 12 hrs after transient treatment with PMA as in Figure 2 followed by cytoskeletal fractionation. The soluble actin in the supernatant (S) represents G-actin, and the insoluble actin in the pellet (P) corresponds with F-actin. n=3 batches of cells; * p < 0.05 vs. DMSO.