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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: J Mol Cell Cardiol. 2018 Jan 4;115:104–114. doi: 10.1016/j.yjmcc.2018.01.003

Figure 6. PKC activation mediates T-tubule remodeling through biphasic cytoskeletal rearrangement.

Figure 6

A, Analysis of F-actin biphasic rearrangement by transiently treating cardiomyocytes with F-actin disruptors (Lat-A and Y-27632) or stabilizer (Cyto-D). Left blot demonstrates that a change in the G-actin/F-actin ratio at 1 hr after treatment. Right blot demonstrates that, 24 hours after washout of the reagents, the G-actin/F-actin ratio returned to baseline. B–E, Summary data of global T-tubule density (B, D) and index of T-tubule integrity (TTint, C, E) in cardiomyocytes transiently (1h treatment, 24h washout) or chronically (24h treatment) treated with cytoskeletal stabilizer CytoD (B, C) or disrupters (Lat-A or Y-27632, D, E). In B and C, n=24, 36 and 31 cells per group, respectively. In D and E, n= 33, 30, 31, 30 and 31 cells per group, respectively. ** p <0.01.