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. Author manuscript; available in PMC: 2018 Mar 6.
Published in final edited form as: Cell Host Microbe. 2017 Sep 21;22(4):443–448.e5. doi: 10.1016/j.chom.2017.08.007

Figure 1. Colonization of Muc2-Deficient Mice with ETBF Results in Lethal BFT-Dependent Disease.

Figure 1

(A and B) Mice were pretreated with antibiotics for 7 days prior to orogastric gavage with ETBF ATCC 43859 WT (A) or Δbft (B) and continued on antibiotics for the duration of the experiment. At 21 days post inoculation the mice were euthanized, the colons fixed, and the tissue stained with H&E. Feces were collected and plated for CFU, demonstrating no significant difference in colonization (WT: 1.36 × 1010 ± 8.51 × 109 CFU/g feces; Δbft: 1.40 × 1010 ± 8.26 × 109). Data are representative of three independent experiments with five mice in each group. Scale bars, 100 µm.

(C and D) Mice were pretreated with antibiotics 24 hr prior to orogastric gavage with ETBF ATCC 43859 WT (A) or Δbft (B) and the antibiotics held thereafter. At 21 days post inoculation the mice were euthanized, the colons fixed, and the tissue stained with H&E. Feces were collected and plated for CFU, demonstrating no significant difference in colonization (WT: 8.00 × 109 ± 3.38 × 109 CFU/g feces; Δbft: 6.56 × 109 ± 4.17 × 109). Data are representative of three independent experiments with five mice in each group. Scale bars, 100 µm. p Values were calculated by one-way ANOVA comparing fecal CFU corresponding to panels (A) to (D).

(E and F) Survival of WT (E) or Muc2−/− mice (F) after orogastric gavage with ETBF ATCC 43859 WT (closed squares) or ETBF Δbft (open squares) clones at 120 hr post colonization.

Results are representative of three independent experiments (E) or are a pooling of three independent experiments (F). Group sizes were as follows: WT mice, ETBF WT (E, closed squares, n = 5); WT mice, ETBF Δbft (E, open squares, n = 5); Muc2−/− mice, ETBF WT (F, closed squares, n = 9); Muc2−/− mice, ETBF Δbft (F, open squares, n = 10). p Values were calculated by log-rank Mantel-Cox test comparing ETBF WT and Δbft groups.