Figure 2. Immunoreactivity of microglial markers in the Mocha cell line.
Mocha cells were immunostained as described in Methods. Immunoreactivity was seen for the 12S E1A immortalizing gene, CD68, Sox10, CD11b, CD40, Iba1, TMEM119, IL-1beta, CD45 and F4/80. Arrows indicate examples of immunoreactive cells. Neuronal beta tubulin and negative control panels show only blue nuclei detected by DAPI. Scale bar = 10 microns.