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. Author manuscript; available in PMC: 2018 Mar 6.
Published in final edited form as: Cell Rep. 2018 Feb 6;22(6):1392–1400. doi: 10.1016/j.celrep.2018.01.035

Figure 5. Effect of Ceramide on Pma1 Activity and on Cell Growth.

Figure 5

(A) Pma1 proton pump activity of WT, Δcer1, Δgcs1, Δgcs1+AbA, Δgcs1+AbA+C6 phytoceramide, and Δgcs1+AbA+C18 ceramide measured by glucose-dependent medium acidification. Data are expressed as mean ± SEM.

(B) In vitro growth of WT, GAL7::IPC1, GAL7:: IPC1+C6 phytoceramide, GAL7::IPC1+ C18 ceramide, and Δcer1 at 37°C, 5% CO2, pH 4.0. Data are expressed as mean ± SEM.