Figure 3. The Nf1 haploinsufficient bone marrow compartment is sufficient to accelerate MPNST formation.

(A) Schematic of bone marrow transplant. Recipient mice were all NI^F/F and received either NI^F/F or NI^F/− bone marrow containing a fluorescent reporter. After 4 weeks of recovery, the mice were injected with Ad-Cre into the sciatic nerve and monitored for MPNST development. (B). Mice that received NI^F/− bone marrow developed MPNST earlier than mice that received NI^F/F bone marrow. (C–E) MPNSTs from mice that received NI^F/− bone marrow showed similar levels of Ki67 staining as mice that received NI^F/F bone marrow, but NI^F/− MPNSTs were enriched in CD45+ cells. Scale bar is equal to 100 microns.