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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: IEEE Trans Image Process. 2018 Apr;27(4):1847–1861. doi: 10.1109/TIP.2017.2787625

Fig. 2.

Fig. 2

The full required imaging system has three stages: patch tracking, deconvolution, and segmentation. In patch tracking, a user-defined template is provided for template matching and tracking spatial coordinates over time with a Kalman filter. In deconvolution (the main focus of this paper), the patch is deconvolved to infer the OPL approximation. In segmentation, a global threshold is applied (using Ostu’s method [45]) to yield a binary segmentation mask to determine the cell membrane location.