Fig. 2.

T-oligo induces expression of p53 and its homologue, p73. SW1573 and H358 cell lysates were prepared 12 and 24 h after exposure to 40 µM T-oligo, and subsequently immunoblotted. (A) SW1573 cells treated with T-oligo exhibited upregulation of p53 (2.5-fold increase) and activated phospho-p53 (Ser-15) (2-fold increase) at 12 h. (B) Upregulation of p21 (1.5-fold increase) was seen at 24 h in addition to an upregulation of E2F1 in SW1573 cells (2-fold increase). (C) H358 cells which have a homozygous deletion of p53, exhibit upregulation of p73 (2.5-fold increase), a p53 homologue 12 h after treatment with T-oligo.