Fig. 6.

T-oligo induces senescence in vivo and in vitro through p33, p27^kip1, pRb, p16 and p21. H358 and SW1573 NSCLC cell lysates and tumor sections were evaluated for a change in expression of senescence inducing proteins by immunoblotting. (A) Treatment with T-oligo in vitro increased the expression of p33 at 48 (2.2-fold) and 96 h (1.6-fold) in H358 cells and increased the expression of p27^kip1 (1.8-fold), pRb (3-fold), p16 (2-fold) and p21 (2-fold) at 48 h. (B) T-oligo induced an upregulation in p33 expression in vitro in SW1573 cells at 96 h (2-fold), and an increase in expression of both p27^kip1 (1.8-fold), and p21 at 72 h (2-fold). (C) Paraffin sections of SW1573 tumors were stained using the M.O.M. kit (Vector) with antibodies against p21, p27^kip1, and p33. Tumor sections treated with complementary oligonucleotide showed no significant expression of p21, p27^kip1 or p33, while tumors treated with T-oligo show marked increase in expression of p21, p27^kip1, and p33 as indicated by the light brown staining.