Table 1.
Antibodies information
| Peptide/protein target | Antigen sequence (if known) | Name of Antibody | Manufacturer, catalog #, and/or name of individual providing the antibody | Species raised in; monoclonal or polyclonal | Dilution used | RRID (required in revised MSs) |
|---|---|---|---|---|---|---|
| AKTS473 | Unknown | Phospho-Akt (Ser473)(D9E) Ab | Cell Signaling (#4060) | Rabbit; monoclonal | 1:2000 | AB_2315049 |
| AKTT308 | Unknown | Phospho-Akt (Thr308) Ab | Cell Signaling (#9275) | Rabbit; monoclonal | 1:1000 | AB_329828 |
| AKT | Unknown | Akt Antibody | Cell Signaling (#9272) | Rabbit; monoclonal | 1:1000 | AB_329827 |
| pS6K1 | Unknown | Phospho-p70 S6 Kinase (Thr389)(108D2) Ab | Cell Signaling (#9234) | Rabbit; monoclonal | 1:1000 | AB_2269803 |
| S6K1 | Unknown | p70 S6 Kinase(49D7) Ab | Cell Signaling (#2708) | Rabbit; monoclonal | 1:1000 | AB_390722 |
| pS6 | Unknown | Phospho-S6 Ribosomal Protein (Ser235/236)(D57.2.2E) Ab | Cell Signaling (#4858) | Rabbit; monoclonal | 1:2000 | AB_916156 |
| S6 | Unknown | S6 Ribosomal Protein (5G10) Ab | Cell Signaling (#2217) | Rabbit; monoclonal | 1:1000 | AB_331355 |
| IRS-1S1101 | Unknown | Phospho-IRS-1 (Ser1101) Antibody | Cell Signaling (#2385) | Rabbit; monoclonal | 1:1000 | AB_330363 |
| IRS-1S636 | Unknown | Phospho-IRS-1 (Ser636/639) Antibody | Cell Signaling (#2388) | Rabbit; monoclonal | 1:1000 | AB_330339 |
| IRS-1 | Unknown | IRS-1 (59G8) Ab | Cell Signaling (#2390) | Rabbit; monoclonal | 1:1000 | AB_561122 |
| pIR | Unknown | Phospho-IGF-I Receptor β (Tyr1131)/Insulin Receptor β (Tyr1146) Antibody | Cell Signaling (#3021) | Rabbit; monoclonal | 1:1000 | AB_331578 |
| IR | Unknown | Insulin Receptor β (4B8) Ab | Cell Signaling (#3025) | Rabbit; monoclonal | 1:1000 | AB_2280448 |
| P85α | Unknown | PI3 Kinase p85 (19H8) Ab | Cell Signaling (#4257) | Rabbit; monoclonal | WB;1:1000 IP;1:50 | AB_10831521 |
| Actin | Unknown | β-Actin Antibody (C4) | Santa Cruz Biotechnology(sc-47778) | Mouse;monoclonal | 1:200 | AB_626632 |