Table 1. Experimental design for L. v. alpina clinical survival experiment, Experiment A.
Population (total number frogs) * | Clutch (total number frogs) * | Exposure group † | Total number of frogs | Number of males ‡ | Number of females ‡ | Number with undetermined gender |
---|---|---|---|---|---|---|
Numbers of L. v. alpina frogs from each population and clutch are outlined, including details of blind randomized block design used for allocation of treatment groups (exposed frogs versus sham-exposed negative control frogs). |
||||||
Eucumbene (99) | A (30) | E | 20 | 7 | 13 | 0 |
C | 10 | 6 | 4 | 0 | ||
B (19) | E | 19 | 10 | 9 | 0 | |
C | 0 | 0 | 0 | 0 | ||
C (29) | E | 20 | 10 | 10 | 0 | |
C | 9 | 4 | 5 | 0 | ||
D (21) | E | 19 | 11 | 8 | 0 | |
C | 2§ | 0 | 1 | 1 | ||
Grey Mare (80) | A (14) | E | 14 | 7 | 6 | 1 |
C | 0 | 0 | 0 | 0 | ||
B (26) | E | 20 | 10 | 10 | 0 | |
C | 6 | 3 | 3 | 0 | ||
C (29) | E | 20 | 11 | 9 | 0 | |
C | 9 | 6 | 3 | 0 | ||
D (11) | E | 11 | 9 | 2 | 0 | |
C | 0 | 0 | 0 | 0 | ||
Kiandra (100) | A (25) | E | 20 | 9 | 11 | 0 |
C | 5 | 1 | 4 | 0 | ||
B (25) | E | 20 | 12 | 8 | 0 | |
C | 5 | 1 | 4 | 0 | ||
C (25) | E | 20| | 8 | 10 | 2 | |
C | 5 | 3 | 2 | 0 | ||
D (25) | E | 20 | 11 | 9 | 0 | |
C | 5 | 1 | 4 | 0 | ||
Ogilvies (76) | A (19) | E | 19 | 13 | 6 | 0 |
C | 0 | 0 | 0 | 0 | ||
B (40) | E | 20 | 7 | 13 | 0 | |
C | 20 | 6 | 14 | 0 | ||
C (17) | E | 16 | 9 | 7 | 0 | |
C | 1¶ | 0 | 0 | 1 |
*Total number of frogs in parentheses for each group. † E represents exposed frogs, C represents control frogs. ‡Gender as determined by post-mortem coelomic examination. §Includes one frog that died pre-exposure and was excluded from data records. |This number includes a frog that died of anasarca post-exposure, unrelated to chytridiomycosis, data still included (frog ID: Lva162). ¶This frog died pre-exposure and was excluded from data records.