Figure 5.

Effect of pupillary constriction on ChR2-mediated light sensitivity in OMR. (A) Representative images showing marked pupillary constriction evoked in DKO mice. Pupillary constriction was blocked by atropine. Light intensity: 3 × 10¹⁵ photons/cm²s with blue LED illumination (470 nm). (B) Statistical data for the normalized pupil area from four DKO mice with and without atropine. Data are expressed as the mean ± SD, n = 4 eyes. (C) Relationships between the threshold light intensity required to produce an OMR and the spatial frequency in DKO mice with and without atropine. Data are expressed as the mean ± SD, n = 4 mice.