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. Author manuscript; available in PMC: 2018 Jun 29.
Published in final edited form as: Cell. 2017 Jun 22;170(1):185–198.e16. doi: 10.1016/j.cell.2017.05.034

Figure 7. Enterochromaffin cells induce mechanical hypersensitivity of colonic afferents.

Figure 7

A. Representative mechanical responses from single low-threshold mechanoreceptive mucosal afferent fibers elicited by a 10mg von Frey hair stimulus to epithelium. Mechanical responses were enhanced following epithelial treatment with norepinephrine (NE, 1µM) and hypersensitivity was blocked by the TRPC4 inhibitor ML204 (10µM) or 5HT3R antagonist alosetron (10µM). n=8–9 per condition. Scale bars: 400µV, 10s. p<0.0001 for contribution of treatment to series variance for NE versus basal and no significant difference with NE+ML204 or alosetron, two-way ANOVA with post-hoc Bonferroni test.

B. Afferent mechanosensory responses were enhanced following epithelial treatment with isovalerate (IVL, 200µM) and hypersensitivity was blocked by alosetron (10µM). A 500mg von Frey hair was used as an epithelial mechanical stimulus for representative traces. n=8–9 per condition. Scale bars: 500µV, 10s. p<0.0001 for contribution of treatment to series variance for IVL versus basal and no significant difference with IVL+alosetron, two-way ANOVA with post-hoc Bonferroni test. All data represented as mean ± sem.