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. 2017 Nov 27;9(11):9618–9631. doi: 10.18632/oncotarget.22709

Figure 4. TDRD9 depletion impairs proliferation in TDRD9-positive cell lines.

Figure 4

(A) Expression levels of TDRD9 in H1299 and H1993 cell lines after the transfection with siRNA control or two different siRNAs against TDRD9. Data are the mean of at least n = 6 qPCR reactions from three independent experiments. Error bars represent ± SD values. (B) Growth curve of TDRD9-depleted cell lines. A TDRD9-negative lung carcinoma cell line (H1264) was used as a control. Data are the average of four (H1299 and H1993) or three (H1264) independent experiments. Error bars represent standard deviation. Levels of TDRD9 mRNA during time points of the growth curve is shown in Supplementary Figure 5. (C) Flow cytometry of TDRD9-positive (H1299, H1993, and H441) or TDRD9-negative (H1264) lung carcinoma cell lines 72 hours after transfection of control siRNA or siRNA against TDRD9. Representative experiments are shown. (D) Level of apoptosis was determined 72 hours after transfection of control siRNA or siRNA against TDRD9, by measuring the percentage of cells containing a subG1 DNA content by flow cytometry. Data are the average of three independent experiments. Error bars represent ± SD values. A, B, D. Significance respect to the siControl was tested by using Student’s t-test. *P < 0.05; **P < 0.01.