Figure 4. Enhanced NFAT signaling induces apoptosis in Il2^−/− immature erythrocytes.

(A) Cell death analysis in BM and splenic Ter119⁺ cells from WT and Il2^−/− mice as revealed by Annexin V staining. (B) Quantification of % Annexin V positive cells in BM CD71⁺Ter119^-, CD71⁺Ter119⁺ and CD71^-Ter119⁺ fractions from WT and Il2^−/− mice. (C) Expression of apoptosis-related genes in the BM Ter119⁺ cells from Il2^−/− mice compared to WT controls. Data in (A–C) are representative of three independent experiments. Number inside each histogram represents percent Annexin V positive population. Data in (B) are presented as mean ± s.d., ns = not significant and ^*p = 0.7376 or 0.0141, paired t-test.