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. 2017 Dec 26;9(11):9672–9684. doi: 10.18632/oncotarget.23822

Figure 5. C/EBPβ transcription factor regulates expression of TMIGD1.

Figure 5

(A) Shown is the schematic of full-length TMIGD1 promoter cloned into pHAGE-GFP reporter vector. (B) HEK-293T cells were transfected with TMIGD1- pHAGE-GFP or EFα1-pHAGE-GFP. Images were taken under a fluorescent microscope (20X) after 48 hours of transfection. (C) TMIGD1 and control EFα1 promoter activities was quantified by FACS analysis via measuring expression of GFP. (D) 5’ biotinylated TMIGD1 promoter mixed either with the cell lysates of HEK-293 cells over-expressing C/EBPβ (LAP) or empty vector and subjected to an electrophoretic mobility shift (EMSA) assay. (E) HEK-293T cells were transfected with full-length (FL) TMIGD1 promoter alone or with C/EBPβ (LAP). After 4 days transfection, cells were prepared and subjected to FACS analysis. The data presented in this figure were independently repeated at least three times.