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. 2018 Jan 19;9(11):9808–9824. doi: 10.18632/oncotarget.24275

Figure 5. RRD-251 increases the D3-induced respiratory burst and oxidative metabolism.

Figure 5

(A) Respiratory burst from inducible oxidative metabolism, measured by flow cytometry of DCF stained cells at 72 hours post treatment, is significantly enhanced with combined RRD-251 plus D3 treatment over D3 alone (D3 vs. RRD-251 + D3 p=0.0069) C DMSO is D3 untreated control where DMSO is the control carrier blank for TPA, C TPA is D3 untreated control cells with the ROS inducible oxidative metabolism inducer, D3 DMSO is D3 treated but ROS measured with only the carrier blank sans TPA, D3 TPA is D3 treated and TPA induced, RRD indicates the parallel cases for RRD-251 treated cells, and RRD D3 indicates the parallel cases for RRD-251 plus D3 treated cells. (B) Expression of p47phox a subunit of NADPH Oxidase was measured by Western blotting at 48 hours for untreated control, and D3 and RRD-251 plus D3 treated cells. RRD-251 significantly increases the D3-induced expression of p47phox. All the experiments were performed in triplicate (three biological repeats).