Figure 10. Colony formation assays.
(A) Colony Formation Assay: Microscopic appearance of NGP cell colonies treated with vehicle and increasing concentrations of HCI-2509 photographed at 2X and 20X magnification. (B) Bar graph representing the decrease on colony numbers on treatment as compared to control using Graphpad prizm. Error bars represent the standard error of mean (SEM) in duplicate assays. Statistical significance calculated by the unpaired student t test comparing the treated wells to vehicle wells. (C) Colony Formation Assay: Microscopic appearance of SK-N-SH cell colonies treated with vehicle and increasing concentrations of HCI-2509 photographed at 2X and 40X magnification. (D) Bar graph representing the decrease on colony numbers as compared to control using Graphpad prizm. Error bars represent the standard error of mean (SEM) in duplicate assays. Statistical significance calculated by the unpaired student t test comparing the treated wells to vehicle wells.