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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Biochim Biophys Acta. 2018 Jan 31;1863(4):467–478. doi: 10.1016/j.bbalip.2018.01.011

Figure 4. Cold exposure triggers lipid depletion and increases acyl-carnitines in the BAT of Lal-/- mice.

Figure 4

(A) Representative electron micrographs from BAT of mice exposed to cold for 4h. Scale bar, 1 μm; LD indicate cytosolic lipid droplets, white arrows indicate LD phospholipid monolayer, black arrows indicate electron-dense phospholipid bilayer of lysosomes. (B) Representative immunofluorescence micrographs of BAT using perilipin 1 as LD marker and cathepsin D as lysosomal marker. (C) Concentrations of acyl-CoAs and (D) carnitine esters (n=4-5). (E) mRNA expression of genes involved in FA mitochondrial trafficking and oxidation, relative to cyclophilin A expression as reference gene. Expression profiles were determined using the 2-ΔΔCt method (n=3). Data represent mean + SEM; p < 0.05 (*), p ≤ 0.01 (**), p ≤ 0.001 (***). Student’s unpaired t test. B, E: male mice; A, C, D: female mice.

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