Skip to main content
. 2017 Apr 6;26(2):146–156. doi: 10.4062/biomolther.2016.272

Fig. 6.

Fig. 6.

Protective effect of spermidine on LPS-induced NO and ROS generation in zebrafish larvae. The zebrafish larvae were treated with 800 μM spermidine and 10 μg/mL LPS for 24 or pretreated with 800 μM spermidine for 1 h prior to incubation with 10 μg/mL LPS for 24 h. (A, B) The levels of NO and ROS generation were observed under a fluorescence microscope after staining with DAF-FM-DA and DCF-DA, respectively. (C and D) The fluorescence intensities of NO and ROS levels in individual zebrafish larvae were quantified, and values represent the means ± SD of three independent experiments (#p<0.05 compared to the control; *p<0.05 compared to the LPS-treated group).