Fig. 7.

Attenuation of LPS-induced recruitment of neutrophils and macrophages by spermidine in zebrafish larvae. After incubation with or without 800 μM spermidine, the larvae were collected, and 10 μg/mL LPS was injected into the yolk using a microinjector. The larvae were stained sudan black or neutral red to detect neutrophils (A) and macrophages (B) migration, respectively. After staining, recruitment of neutrophils and macrophages was observed using a dissecting microscope. The representative pictures for neutrophils and macrophages (Control, PBS-injected negative controls; LPS, LPS-injected positive controls; spermidine + LPS, spermidine-treated and LPS-injected larvae) are shown.