Fig. 9.

Effect of diosmetin on NLRP3 inflammasome protein expression in vitro. (A, B) RAW264.7 and A549 cells were pre-treated with diosmetin (40 μM) for 1 h, followed by stimulation with LPS (1 μg/ml) for 6 h and ATP (5 mM) for 40 min, and then protein expression of NLRP3 was determined by western blot in RAW264.7 (A) and A549 (B) cells. (C, D) Densitometry quantitation of protein expression in RAW264.7 (C) and A549 (D) cells. β-actin acted as an internal control. Data was expressed as means ± SEM. n=5. ^**p<0.01 vs the control group. ^##p<0.01 vs the LPS plus ATP group.