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. Author manuscript; available in PMC: 2019 Jan 11.
Published in final edited form as: Cell. 2017 Dec 28;172(1-2):331–343.e13. doi: 10.1016/j.cell.2017.12.008

Figure 7. Cdc13EBM-C Has an Activation Effect on Telomerase.

Figure 7

(A) Mutations in Cdc13EBM-C (cdc13E252K, cdc13F253A and cdc13S255A) exhibited critically short telomeres. Cells were taken from serial streaks 2, 4 and 6 after sporulation. Vertical white spaces between lanes 1 and 2, 7 and 8, indicate cropping of superfluous lanes. (B) Strains as described in (A) were streaked onto plates to analyze senescence phenotype. (C) Fusion of Cdc13-Est2 cannot fully bypass the role of Est1. A diploid CDC13/cdc13Δ EST1/est1Δ strain was cotransformed with plasmids expressing Cdc13-Est2 (or Cdc13–2-Est2) and Est1 (or Est1–60). After sporulation, cells were taken from streak 6 and subjected to southern blot analysis. (D) A schematic model for telomerase recruitment to telomeres in budding yeast.