Table 1. Gene name, gene symbol, GenBank accession numbers, primer sequences, primer location, annealing temperature (Ta) and amplicon length for each evaluated RGs.
Gene Name | Gene Symbol | Accession Number | Primers 5'-3' (Forward, Reverse) | Primer Location | Ta | Amplicon Size (bp) |
---|---|---|---|---|---|---|
β- actin | ACTB | AY141970 |
GCGTGGCTACAGCTTCACC TTGATGTCACGGACGATTTC |
EXON 4 | 60°C | 56 |
Glyceraldehyde 3-phosphate dehydrogenase | GAPDH | BC102589 |
TGGAAAGGCCATCACCATCT CCCACTTGATGTTGGCAG |
EXON 4–5 | 60°C | 60 |
Eukaryotic translation elongation factor 1 alpha 1 | EEF1A1 | BC105315 |
CATCCCAGGCTGACTGTGC TGTAAGCCAAAAGGGCATGC |
EXON 3–4 | 60°C | 101 |
β2 Microglobulin | B2M | NM_173893 |
CTGCTATGTGTATGGGTTCC GGAGTGAACTCAGCGTG |
EXON 2 | 60°C | 101 |
Hydroxymethylbilane synthase | HMBS | BC112573.1 | CTTTGGAGAGGAATGAAGTGG AATGGTGAAGCCAGGAGGAA | EXON 5–6 | 60°C | 80 |
Ribosomal protein L4 | RPL4 | NM_001014894 | TTGGAAACATGTGTCGTGGG GCAGATGGCGTATCGCTTCT | EXON 3–4 | 60°C | 101 |
Ribosomal protein S15 | RPS15 | BC108231 |
GAATGGTGCGCATGAATGTC GACTTTGGAGCACGGCCTAA |
EXON 2 | 60°C | 101 |
Ribosomal protein S23 | RPS23 | BC102049 |
CCCAATGATGGTTGCTTGAA CGGACTCCAGGAATGTCACC |
EXON 3–4 | 60°C | 101 |
Ribosomal protein S9 | RPS9 | DT860044 |
CCTCGACCAAGAGCTGAAG CCTCCAGACCTCACGTTTGTTC |
EXON 2–3 | 60°C | 54 |
Ubiquitously expressed transcript |
UXT | CR452243 |
TGTGGCCCTTGGATATGGTT GGTTGTCGCTGAGCTCTGTG |
EXON 5–6 | 60°C | 101 |