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. 2018 Mar 6;13(3):e0191558. doi: 10.1371/journal.pone.0191558

Table 1. Gene name, gene symbol, GenBank accession numbers, primer sequences, primer location, annealing temperature (Ta) and amplicon length for each evaluated RGs.

Gene Name Gene Symbol Accession Number Primers 5'-3' (Forward, Reverse) Primer Location Ta Amplicon Size (bp)
β- actin ACTB AY141970 GCGTGGCTACAGCTTCACC
TTGATGTCACGGACGATTTC
EXON 4 60°C 56
Glyceraldehyde 3-phosphate dehydrogenase GAPDH BC102589 TGGAAAGGCCATCACCATCT
CCCACTTGATGTTGGCAG
EXON 4–5 60°C 60
Eukaryotic translation elongation factor 1 alpha 1 EEF1A1 BC105315 CATCCCAGGCTGACTGTGC
TGTAAGCCAAAAGGGCATGC
EXON 3–4 60°C 101
β2 Microglobulin B2M NM_173893 CTGCTATGTGTATGGGTTCC
GGAGTGAACTCAGCGTG
EXON 2 60°C 101
Hydroxymethylbilane synthase HMBS BC112573.1 CTTTGGAGAGGAATGAAGTGG AATGGTGAAGCCAGGAGGAA EXON 5–6 60°C 80
Ribosomal protein L4 RPL4 NM_001014894 TTGGAAACATGTGTCGTGGG GCAGATGGCGTATCGCTTCT EXON 3–4 60°C 101
Ribosomal protein S15 RPS15 BC108231 GAATGGTGCGCATGAATGTC
GACTTTGGAGCACGGCCTAA
EXON 2 60°C 101
Ribosomal protein S23 RPS23 BC102049 CCCAATGATGGTTGCTTGAA
CGGACTCCAGGAATGTCACC
EXON 3–4 60°C 101
Ribosomal protein S9 RPS9 DT860044 CCTCGACCAAGAGCTGAAG
CCTCCAGACCTCACGTTTGTTC
EXON 2–3 60°C 54
Ubiquitously
expressed transcript
UXT CR452243 TGTGGCCCTTGGATATGGTT
GGTTGTCGCTGAGCTCTGTG
EXON 5–6 60°C 101