Table 3. Parameters based quantitative cycle (Cq) values for 10 candidate RGs.
| ACTB | GAPDH | RPL4 | EEF1A1 | RPS15 | RPS23 | RPS9 | HMBS | B2M | UXT | |
|---|---|---|---|---|---|---|---|---|---|---|
| N | 54 | 54 | 54 | 54 | 54 | 54 | 54 | 54 | 54 | 54 |
| geo Mean [Cq] | 20.68 | 20.30 | 20.81 | 19.94 | 22.69 | 19.11 | 19.74 | 25.44 | 19.69 | 23.93 |
| ar Mean [Cq] | 20.72 | 20.35 | 20.85 | 19.99 | 22.80 | 19.15 | 19.76 | 25.50 | 19.81 | 23.96 |
| min [Cq] | 17.82 | 16.32 | 18.28 | 17.21 | 19.15 | 16.41 | 18.07 | 20.47 | 15.29 | 21.99 |
| max [Cq] | 24.82 | 22.83 | 23.67 | 23.46 | 27.99 | 21.72 | 23.80 | 28.20 | 26.11 | 27.97 |
| Std dev [±Cq] | 1.13 | 1.11 | 0.97 | 1.22 | 1.89 | 0.97 | 0.75 | 1.34 | 1.63 | 0.95 |
| CV [%Cq] | 5.44 | 5.46 | 4.64 | 6.08 | 8.30 | 5.08 | 3.78 | 5.25 | 8.25 | 3.95 |
| min [x-fold] | -7.25 | -15.71 | -5.78 | -6.63 | -11.64 | -6.48 | -3.18 | -31.34 | -21.09 | -3.83 |
| max [x-fold] | 17.68 | 5.79 | 7.23 | 11.49 | 39.42 | 6.12 | 16.62 | 6.79 | 86.01 | 16.49 |
| Std dev [± x-fold] |
2.19 | 2.16 | 1.96 | 2.32 | 3.71 | 1.96 | 1.68 | 2.53 | 3.11 | 1.93 |
N = number of samples, geo Mean[Cq] = geometric mean of Cq; ar Mean[Cq] = arithmetic mean of Cq; min [Cq] and max [Cq] = extreme values of Cq; Std dev [±Cq] = standard deviation of the Cq; CV [%Cq] = coefficient of variation expressed as a percentage on the Cq values; min [x-fold] and max [x-fold] = extreme values of expression levels expressed as absolute x-fold over or under coefficient; std dev[±x-fold] = standard deviation of the absolute regulation coefficients.