Table I.

Results of the qPCR and WB assays in 20 asymptomatic Leishmania infantum-infected blood donors at different intervals during the study period.

Donors	Age (years)	Gender	Date	Western blot	Peripheral blood qPCR	Contact with animals
Donor 1	52	Male	October-08	POS 16	POS*	Daily contact with dogs (a dog at home)
			November-10	POS 16	POS*	Had a dog that died of leishmaniasis
			March-11	POS 14, 16	POS*
Donor 2	44	Female	October-08	POS 14, 16	POS*	Daily contact with dogs with leishmaniasis
			November-10	NEG	POS*	Works as a veterinarian
Donor 3	26	Male	October-08	NEG	POS*	Daily contact with dogs (a dog at home)
			March-10	NEG	NEG
Donor 4	28	Male	November-08	NEG	POS*	Daily contact with dogs (a dog at home)
			March-10	NEG	NEG
Donor 5	49	Male	January-09	NEG	POS*	Daily contact with dogs (two dogs at home)
			March-10	NEG	NEG	Had a dog that died of leishmaniasis
Donor 6	35	Female	February-09	NEG	POS**	Daily contact with dogs (a dog at home)
			June-09	POS 14, 16	NEG	Works as a housekeeper in a country house with dogs
			November-10	NEG	NEG
Donor 7	55	Male	February-09	NEG	POS*	No data
			March-10	NEG	NEG
Donor 8	40	Male	February-09	NEG	POS**	Daily contact with dogs (a dog and a cat at home)
			May-09	NEG	NEG
			November-10	NEG	POS**
			March-11	NEG	POS**
Donor 9	47	Male	June-09	POS 14, 16	POS*	Daily contact with dogs (a dog at home)
			March-10	POS 14, 16	NEG
			October-10	POS 14, 16	POS*
Donor 10	55	Male	October-09	POS 14, 16	NEG	Currently no contact with dogs
			January-10	POS 14, 16	POS*	Owned a dog in the past
Donor 11	49	Male	August-09	POS 16 w	POS*	Daily contact with dogs (a dog at home)
			October-09	POS 14	NEG	Had a dog that died of leishmaniasis
Donor 12	60	Male	August-09	POS 14, 16	NEG	No contact with dogs (a cat at home)
			October-09	POS 14, 16	NEG
Donor 13	42	Female	August-09	POS 14, 16	NEG	Daily contact with dogs (a dog at home)
			October-09	POS 14, 16	NEG	Had a dog that died of leishmaniasis
Donor 14	43	Male	October-11	NEG	POS*	No data
Donor 15	43	Male	September-11	NEG	POS*	No dog at home
Donor 16	55	Female	June-11	POS 14	POS*	Had three dogs that died of leishmaniasis
Donor 17	49	Male	October-08	POS 14, 16	NEG	Occasional contact with dogs
			July-11	POS 14, 16	NEG
Donor 18	33	Male	September-11	POS 14	NEG	No dog at home
						Family dog died of leishmaniasis
Donor 19	64	Male	October-11	POS 14	NEG	Daily contact with dogs (two dogs at home)
Donor 20	42	Female	October-11	POS 14, 16	NEG	Daily contact with dogs (two dogs at home)

qPCR: quantitative polymerase chain reaction; WB: western blot; POS*: ≤0.1 parasite equivalent/mL; POS**: 1 parasite equivalent/mL; POS 14, 16: WB positive with 14 and 16 kDa bands; POS 14: WB positive with a 14 kDa band; POS 16: WB positive with a 16 kDa band; POS 16 w: WB weakly positive with 16 kDa band.