Figure 9. HOCl oxidizes roGFP2-based probes in E. coli effectively and with fast kinetics.

All three roGFP2-based probes used in this study, roGFP2-Orp1 (A), Grx1-roGFP2 (B), and unfused roGFP2(C) are oxidized within mixing time upon addition of HOCl. E. coli cells expressing the respective probes were incubated in a 96-well plate reader and a time-course measurement of the ratio of the fluorescence intensity of the probe at excitation wavelengths of 405 and 488 nm was performed. The arrow indicates the addition of different concentrations of HOCl or medium as a control as indicated.