Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Chemical compound, drug | PTX | Enzo | Cat#:BML-G100 | 100 ng/ml |
| Chemical compound, drug | U0126 | Sigma | Cat#:U120 | 10 μM |
| Chemical compound, drug | Ro 31–8220 | Adooq | Cat#:A13514 | 500 nM |
| Chemical compound, drug | NF449 | Tocris | Cat#:1391 | 1 μM |
| Chemical compound, drug | PKI14-22 | Adooq | Cat#:A16031 | 300 nM |
| Chemical compound, drug | H89 | Beyotime | Cat#:S1643 | 500 nM |
| Chemical compound, drug | bumetanide | Aladdin | Cat#:B129942 | 10 μM |
| Chemical compound, drug | Ani9 | Sigma | Cat#:SML1813 | 150 nM |
| Chemical compound, drug | Niflumic acid (NFA) | Aladdin | Cat#:N129597 | 20 μM |
| Chemical compound, drug | DIDS | Sigma | Cat#:D3514 | 20 μM |
| Chemical compound, drug | GlyH-101 | Adooq | Cat#:A13723 | 10 μM |
| Chemical compound, drug | CFTRinh-172 | Adooq | Cat#:A12897 | 10 μM |
| Chemical compound, drug | EGTA | Aladdin | Cat#:E104434 | 5 mM |
| Chemical compound, drug | SKF96365 | Sigma | Cat#:S7809 | 10 μM |
| Chemical compound, drug | Ruthenium red | Sigma | Cat#:R2751 | 10 μM |
| Chemical compound, drug | Nicardipine | Sigma | Cat#:N7510 | 20 μM |
| Chemical compound, drug | LaCl3 | Sigma | Cat#:449830 | 100 μM |
| Chemical compound, drug | IBMX | Sigma | Cat#:I7018 | 100 μM |
| Chemical compound, drug | U73122 | Sigma | Cat#:U6756 | 10 μM |
| Chemical compound, drug | Forskolin | Beyotime | Cat#:S1612 | 10 μM |
| Chemical compound, drug | PD123319 | Adooq | Cat#:A13201 | 1 μM |
| Chemical compound, drug | Candesartan | Adooq | Cat#:A10175 | 1 μM |
| Chemical compound, drug | Amiloride | Aladdin | Cat#:A129545 | 1 mM |
| Chemical compound, drug | Acetazolamide | Medchem express | Cat#:HY-B0782 | 500 μM |
| Peptide, recombinant protein | ANGII | China Peptides | 100 nM | |
| Commercial assay or kit | Carboxy SNARF−1, acetoxymethyl ester | Invitrogen | Cat#:C-1272 | 5 μM |
| Commercial assay or kit | Lipofectamine TM2000 | Invitrogen | Cat#:11668–019 | |
| Commercial assay or kit | Collagenase I | sigma | Cat#:C0130 | |
| Commercial assay or kit | cAMP ELISA kit | R and D systems | Cat#:KGE012B | |
| Commercial assay or kit | IP1 ELISA assay | Shanghai Lanpai Biotechnology Co., Ltd | Cat#:lp034186 | |
| Commercial assay or kit | The dual-luciferase reporter assay system | Promega | Cat#:E1960 | |
| Antibody | ADGRG2 antibody(rabbit polyclonal) | Sigma | RRID:AB_1078923 | |
| Antibody | ADGRG2 antibody(rabbit polyclonal) | Sigma | RRID:AB_2722557 | |
| Antibody | ADGRG2 antibody(sheep polyclonal) | R and D systems | RRID:AB_2722556 | |
| Antibody | CFTR antibody(goat polyclonal) | Santa Cruz | RRID:AB_638427 | |
| Antibody | CFTR antibody(rabbit polyclonal) | Proteintech | RRID:AB_2722558 | |
| Antibody | Gq antibody(goat polyclonal) | Santa Cruz | RRID:AB_2279038 | |
| Antibody | Gq antibody(rabbit polyclonal) | Proteintech | RRID:AB_2111647 | |
| Antibody | Flag antibody(mouse monoclonal) | Sigma | RRID:AB_259529 | |
| Antibody | HA antibody(mouse monoclonal) | Santa Cruz | RRID:AB_627809 | |
| Antibody | GAPDH(rabbit monoclonal) | Cell Signaling | RRID:AB_10622025 | |
| Antibody | Gs antibody(rabbit polyclonal) | Proteintech | RRID:AB_2111668 | |
| Antibody | Gi antibody(mouse monoclonal) | Santa Cruz | RRID:AB_2722559 | |
| Antibody | β-arrestin-1 antibody(rabbit polyclonal) | Dr R.J. Lefkowitz | A1CT | |
| Antibody | β-arrestin-2 antibody(rabbit polyclonal) | Dr R.J. Lefkowitz | A2CT | |
| Antibody | ANO1 antibody(rabbit polyclonal) | Proteintech | RRID:AB_2722560 | |
| Antibody | Ezrin antibody(rabbit polyclonal) | Proteintech | RRID:AB_2722561 | |
| Antibody | Acetylated Tubulin(Lys40) Antibody(mouse monoclonal) | Proteintech | RRID:AB_2722562 | |
| Antibody | Donkey anti-sheep IgG(H + L) (secondary antibody) | Abcam | RRID:AB_2716768 | |
| Antibody | Donkey anti-rabbit IgG(H + L) (secondary antibody) | Invitrogen | RRID:AB_2534017 | |
| Antibody | Donkey anti-mouse IgG(H + L) (secondary antibody) | Invitrogen | RRID:AB_141607 | |
| Antibody | Donkey anti-goat IgG(H + L) (secondary antibody) | Invitrogen | RRID:AB_142672, RRID:AB_141788 | |
| Antibody | HRP-conjugated Affinipure Rabbit Anti-Sheep IgG(H + L) | Proteintech | RRID:AB_2722563 | |
| Antibody | HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H + L) | Proteintech | RRID:AB_2722564 | |
| Antibody | HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H + L) | Proteintech | RRID:AB_2722565 |
All other chemicals or reagents were from Sigma unless otherwise specified.