Table 1. qPCR primers.
| Name | Forward primer (5′ to 3′) | Reverse primer (5′ to 3′) |
|---|---|---|
| Genomic locus | ||
| RPP38 (ref) | TCATTGGCTTAAAATGTGT | TTTATTTTAAGGGGTTGTAATG |
| RC3H2 (ref) | ACTAAGTCAAGGCTACTGTG | TTCTGGTGTCAGTATGGAAG |
| hTREM2 | ATCCCTGCCCAGTCCACCCTTGATGGCT | CCATCCTTCTCCTCCTGGCCTGCATCTTT |
| hTREML1 | GCGTACGTTTCTCACAGACC | CCCACTCCTCAAGAACCCAT |
| hTREML2 | TTCACCCCAGGACTCATCAC | GGCATGGAGGGTAGTCTGTT |
| Gene | ||
| Actb | GGAGGGGGTTGAGGTGTT | TGTGCACTTTTATTGGTCTCAAG |
| Tyrobp | GAGTGACACTTTCCCAAGATGC | CCTTGACCTCGGGAGACCA |
| hTREM2 iso1 | GCATCTCCAGGAGCCTCTTG | TGAGAAAGATGCAGGCCAGG |
| hTREM2 iso2 | CGGGATGCTGGAGATCTCTG | TCTCAGCCCTGGAGATGCT |
| hTREM2 iso3 | CATCTCCAGGCCATCTCAAG | AGGAGGAGAAGGATGGAAGT |
| hTREML1 | CTGGCGAGTATGGCTGCAT | TGCCAATCTTATGGGTCTCTTCT |
| hTREML2 | CCTATAAGGGCTACAAAAACCGC | CCCATCAAGGGGTACAGGA |
| Spp1 | CACTCCAATCGTCCCTACAGT | CTGGAAACTCCTAGACTTTGACC |
| Gpnmb | CATTCCCATCTCGAAGGTGAAA | AAATGGCAGAGTCGTTGAGGA |
| Cst7 | GGAGCTGTACTTGCCGAGC | CATGGGTGTCAGAAGTTAGGC |
| Tnf | GCCTCTTCTCATTCCTGCTTGT | TGATCTGAGTGTGAGGGTCTGG |
| Il1b | GAAATGCCACCTTTTGACAGTG | TGGATGCTCTCATCAGGACAG |
| Il6 | GCCAGAGTCCTTCAGAGAGATACA | CTTGGTCCTTAGCCACTCCTTC |