Figure 5.

hM4Di prevents the effects of prior ketamine on activation of the PL-DRN pathway. A, Schematic diagram of the injection procedure. Rats were injected with retrogradely transported AAV vectors encoding Cre and eGFP. Five days later, rats were injected with Cre-dependent AAV vectors encoding hM4Di-mCherry Scale bar: 250 μm and applies to both images. B, Representative images of the PL showing hM4Di-mCherry expression (left), Fos expression (middle), and the colocalization of hM4Di-mCherry and Fos (right). Scale bar: 50 μm. C, Number of hM4Di-mCherry labeled cells (left), Fos+ cells (middle), and percentage of hM4Di-mCherry expressing cells that also express Fos (right) in the PL of rats that received ketamine before CNO or vehicle. Unpaired t test: **p < 0.01. Bars represent group mean ± SEM.