Table 1.
| Figure | Data Structure | Type of test | ANOVA p values, t test p values, 95% CI |
|---|---|---|---|
| Fig. 1 | Normal distribution | Two-way ANOVA | Main effect of stress: p < 0.0001 Main effect of drug: p < 0.0001 Interaction: p = 0.0249 |
| Tukey’s multiple comparisons test | |||
| Homecage:saline vs homecage:ketamine (10 mg/kg) | 9−19.55 to 10.49 | ||
| Homecage:saline vs homecage:ketamine (40 mg/kg) | −10.27 to 20.6 | ||
| Homecage:saline vs stress:saline | 7.037 to 37.08 | ||
| Homecage:saline vs stress:ketamine (10 mg/kg) | −17.34 to 12.7 | ||
| Homecage:saline vs stress:ketamine (40 mg/kg) | 6.635 to 37.5 | ||
| Homecage:ketamine (10 mg/kg) vs Homecage:ketamine (40 mg/kg) | −5.735 to 25.13 | ||
| Homecage:ketamine (10 mg/kg) vs stress:saline | 11.57 to 41.61 | ||
| Homecage:ketamine (10 mg/kg) vs stress:ketamine (10 mg/kg) | −12.81 to 17.23 | ||
| Homecage:ketamine (10 mg/kg) vs stress:ketamine (40 mg/kg) | 11.16 to 42.03 | ||
| Homecage:ketamine (40 mg/kg) vs stress:saline | 1.459 to 32.32 | ||
| Homecage:ketamine (40 mg/kg) vs stress:ketamine (10 mg/kg) | −22.92 to 7.942 | ||
| Homecage:ketamine (40 mg/kg) vs stress:ketamine (40 mg/kg) | 1.067 to 32.73 | ||
| Stress:saline vs stress:ketamine (10 mg/kg) | −39.4 to −9.36 | ||
| Stress:saline vs stress:ketamine (40 mg/kg) | −15.42 to 15.44 | ||
| Stress:ketamine (10 mg/kg) vs stress:ketamine (40 mg/kg) | 8.958 to 39.82 | ||
| Fig. 2 | Normal distribution | ANOVA | |
| Total 5-HT Rostral | Two-way ANOVA | Stress: p = 0.16 Drug: p = 0.47 Interaction: p = 0.81 |
|
| Total 5-HT Middle | Two-way ANOVA | Stress: p = 0.59 Drug: p = 0.06 Interaction: p = 0.60 |
|
| Total 5-HT Caudal | Two-way ANOVA | Stress: p = 0.55 Drug: p = 0.23 Interaction: p = 0.16 |
|
| Fig. 2, middle | ANOVA | ||
| Total Fos Rostral | Two-way ANOVA | Stress: p = 0.0004 Drug: p = 0.047 Interaction: p = 0.055 |
|
| Tukey's multiple comparisons test | |||
| Saline:HC vs saline:IS Saline:HC vs ketamine:HC Saline:HC vs ketamine:IS Saline:IS vs ketamine:HC Saline:IS vs ketamine:IS Ketamine:HC vs ketamine:IS |
−25.9 to −5.303 −10.1 to 10.5 −14.76 to 4.959 5.503 to 26.1 0.8412 to 20.56 −14.96 to 4.759 |
||
| Total Fos Middle | Two-way ANOVA | Stress: p = 0.0004 Drug: p = 0.04 Interaction: p = 0.044 |
|
| Tukey's multiple comparisons test | |||
| Saline:HC vs saline:IS Saline:HC vs ketamine:HC Saline:HC vs ketamine:IS Saline:IS vs ketamine:HC Saline:IS vs ketamine:IS Ketamine:HC vs ketamine:IS |
−17.4 to −3.713 −6.544 to 6.744 −9.418 to 3.251 3.526 to 17.78 0.6301 to 14.31 −9.827 to 3.46 |
||
| Total Fos Caudal | Two-way ANOVA | Stress: p = 0.004 Drug: p = 0.62 Interaction: p = 0.83 |
|
| Tukey's multiple comparisons test | |||
| Saline:HC vs saline:IS Saline:HC vs ketamine:HC Saline:HC vs ketamine:IS Saline:IS vs ketamine:HC Saline:IS vs ketamine:IS Ketamine:HC vs ketamine:IS |
−7.269 to 0.5419 −3.602 to 4.209 −6.341 to 1.069 −0.4295 to 7.763 −3.178 to 4.633 −6.845 to 0.9661 |
||
| Fig. 2, right | Normal distribution | ||
| 5-HT + Fos Rostral | Two-way ANOVA | Stress: p < 0.0001 Drug: p = 0.046 Interaction: p = 0.08 |
|
| Tukey's multiple comparisons test | |||
| Saline:HC vs saline:IS Saline:HC vs ketamine:HC Saline:HC vs ketamine:IS Saline:IS vs ketamine:HC Saline:IS vs ketamine:IS Ketamine:HC vs ketamine:IS |
−9.626 to −2.267 −3.43 to 3.929 −5.808 to 1.238 2.516 to 9.876 0.1389 to 7.185 −6.057 to 0.989 |
||
| 5-HT + Fos Middle | Two-way ANOVA | Stress: p < 0.0001 Drug: p = 0.03 Interaction: p = 0.03 |
|
| Tukey's multiple comparisons test | |||
| Saline:HC vs saline:IS Saline:HC vs ketamine:HC Saline:HC vs ketamine:IS Saline:IS vs ketamine:HC Saline:IS vs ketamine:IS Ketamine:HC vs ketamine:IS |
−7.904 to −2.494 −2.625 to 2.628 −4.548 to 0.4605 2.382 to 8.019 0.4502 to 5.86 −4.672 to 0.581 |
||
| 5-HT + Fos Caudal | Two-way ANOVA | Stress: p < 0.0001 Drug: p = 0.03 Interaction: p = 0.03 |
|
| Tukey's multiple comparisons test | |||
| Saline:HC vs saline:IS Saline:HC vs ketamine:HC Saline:HC vs ketamine:IS Saline:IS vs ketamine:HC Saline:IS vs ketamine:IS Ketamine:HC vs ketamine:IS |
−4.4 to −0.994 −1.515 to 1.892 −3.019 to 0.212 1.099 to 4.672 −0.4097 to 2.996 −3.295 to 0.1108 |
||
| Fig. 3 | Normal distribution | ||
| Total RAM | Two-way ANOVA | Stress: p = .99 Drug: p = 0.0003 Interaction: p = 0.87 |
|
| Homecage:saline vs Homecage:ketamine (10 mg/kg) Homecage:saline vs homecage:ketamine (40 mg/kg) Homecage:saline vs stress:saline Homecage:saline vs stress:ketamine (10 mg/kg) Homecage:saline vs stress:ketamine (40 mg/kg) Homecage:ketamine (10 mg/kg) vs homecage:ketamine (40 mg/kg) Homecage:ketamine (10 mg/kg) vs stress:saline Homecage:ketamine (10 mg/kg) vs stress:ketamine (10 mg/kg) Homecage:ketamine (10 mg/kg) vs stress:ketamine (40 mg/kg) Homecage:ketamine (40 mg/kg) vs stress:saline Homecage:ketamine (40 mg/kg) vs stress:ketamine (10 mg/kg) Homecage:ketamine (40 mg/kg) vs stress:ketamine (40 mg/kg) Stress:saline vs stress:ketamine (10 mg/kg) Stress:saline vs stress:ketamine (40 mg/kg) Stress:ketamine (10 mg/kg) vs stress:ketamine (40 mg/kg) |
−46.01 to 13.18 −60.26 to −1.071 −27.01 to 32.18 −50.1 to 9.096 −57.58 to −0.5401 −43.85 to 15.35 −10.6 to 48.6 −33.68 to 25.51 −41.16 to 15.88 3.654 to 62.85 −19.43 to 39.76 −26.91 to 30.13 −52.68 to 6.513 −60.16 to −3.123 −37.08 to 19.96 |
||
| Total Fos | Two-way ANOVA | Stress: p < 0.0001 Drug: p = 0.1326 Interaction: p = 0.1498 |
|
| Homecage:saline vs homecage:ketamine (10 mg/kg) Homecage:saline vs homecage:ketamine (40 mg/kg) Homecage:saline vs stress:saline Homecage:saline vs stress:ketamine (10 mg/kg) Homecage:saline vs stress:ketamine (40 mg/kg) Homecage:ketamine (10 mg/kg) vs homecage:ketamine (40 mg/kg) Homecage:ketamine (10 mg/kg) vs stress:saline Homecage:ketamine (10 mg/kg) vs stress:ketamine (10 mg/kg) Homecage:ketamine (10 mg/kg) vs stress:ketamine (40 mg/kg) Homecage:ketamine (40 mg/kg) vs stress:saline Homecage:ketamine (40 mg/kg) vs stress:ketamine (10 mg/kg) Homecage:ketamine (40 mg/kg) vs stress:ketamine (40 mg/kg) Stress:saline vs stress:ketamine (10 mg/kg) Stress:saline vs stress:ketamine (40 mg/kg) Stress:ketamine (10 mg/kg) vs stress:ketamine (40 mg/kg) |
−27.09 to 12.92 −34.42 to 5.587 −48.67 to −8.663 −59 to −19 −47.66 to −9.105 −27.34 to 12.67 −41.59 to −1.579 −51.92 to −11.91 −40.57 to −2.021 −34.25 to 5.754 −44.59 to −4.579 −33.24 to 5.312 −30.34 to 9.671 −18.99 to 19.56 −8.657 to 29.9 |
||
| % RAM + Fos | Two-way ANOVA | Stress: p = 0.0064 Drug: p < 0.0001 Interaction: p = 0.0128 |
|
| Homecage:saline vs homecage:ketamine (10 mg/kg) Homecage:saline vs homecage:ketamine (40 mg/kg) Homecage:saline vs stress:saline Homecage:saline vs stress:ketamine (10 mg/kg) Homecage:saline vs stress:ketamine (40 mg/kg) Homecage:ketamine (10 mg/kg) vs homecage:ketamine (40 mg/kg) Homecage:ketamine (10 mg/kg) vs stress:saline Homecage:ketamine (10 mg/kg) vs stress:ketamine (10 mg/kg) Homecage:ketamine (10 mg/kg) vs stress:ketamine (40 mg/kg) Homecage:ketamine (40 mg/kg) vs stress:saline Homecage:ketamine (40 mg/kg) vs stress:ketamine (10 mg/kg) Homecage:ketamine (40 mg/kg) vs stress:ketamine (40 mg/kg) |
−21.56 to 5.718 −16.43 to 10.84 −13.64 to 13.64 −40.35 to −13.07 −19.63 to 6.654 −8.516 to 18.76 −5.718 to 21.56 −32.43 to −5.156 −11.71 to 14.57 −10.84 to 16.43 −37.55 to −10.28 −16.83 to 9.452 |
||
| Stress:saline vs stress:ketamine (10 mg/kg) Stress:saline vs stress:ketamine (40 mg/kg) Stress:ketamine (10 mg/kg) vs stress:ketamine (40 mg/kg) |
−40.35 to −13.07 −19.63 to 6.654 7.084 to 33.37 |
||
|
Fig. 4 Total RB |
Normal distribution | Independent samples t test T,df |
p = 0.49 t = 0.70; df = 13 |
|
Fig. 4 Total Fos |
Normal distribution | Independent samples t test T,df |
p = 0.0012 t = 4.145; df = 13 |
|
Fig. 4C % Double Label |
Normal distribution | Independent samples t test T,df |
p = 0.0043 t = 3.453; df = 13 |
| Fig. 5 | Normal distribution | Independent samples t test T,df |
|
| Total hM4Di |
p = 0.83 t = 0.21; df = 8 |
||
| Total Fos |
p = 0.0025 t = 0.4.34; df = 8 |
||
| hM4Di + Fos |
p = 0.0037 t = 4.05; df = 8 |
||
| Fig. 6 | Normal distribution | Three-way ANOVA | p value, power |
| Drug Stress Virus Drug*Stress Drug*Virus Stress*Virus Drug*Stress*Virus |
0.053, 0.47 <0.0001, 1.0 0.085, 0.39 0.004, 0.851 0.18, 0.252 0.0001, 0.989 0.023, 0.59 |