Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Mar 5;217(3):1079–1095. doi: 10.1083/jcb.201610098

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Beati et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 8. — Overexpression of GFP-Smash PM and GFP-Smash PI rescues semilethality of smash³⁵ mutant animals. (A) GFP-Smash constructs used for the rescue assays. + or – in the column to the right indicates whether semilethality of smash³⁵ is rescued. The number of eclosed smash³⁵ homozygous adults expressing the respective GFP-Smash construct under control of the ubiquitous act5C::Gal4 driver line was compared with the number of eclosed smash³⁵ homozygous mutant adults from the same cross carrying only act5C::Gal4 or the respective UAS::GFP-Smash construct as negative control. Rescue was scored (+) when the eclosion rate of smash³⁵ homozygous mutant adults expressing the rescue construct was significantly (P < 0.05) higher than in the negative control. (B) Quantification of the rescue assays. Blue bars, mean percentage of animals with the respective genotype ± SEM; red bars, mean percentage of animals with the respective genotype divided by 2 ± SEM; *, P < 0.05; **, P < 0.01; ns, not significant. GFP-Smash PM: P = 0.041, n = 663; GFP-Smash PJ: P = 0.143, n = 525; GFP-Smash PI: P = 0.014, n = 579; GFP-Smash PI ΔPBM: P = 0.045, n = 626; GFP-Smash PI ΔLIM: P = 0.033, n = 613; GFP-Smash PI N-term: P = 0.071, n = 875; GFP-Smash PI C-term: P = 0.009, n = 1072; GFP-Smash PI YmultiF: P = 0.021, n = 632.