Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Mar 5;217(3):1033–1045. doi: 10.1083/jcb.201705104

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Tsoumpekos et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 6. — Bbg is in the same protein complex as Sqh and stabilizes Sqh in the apical cytocortex. (A and A′) Pouch of en-Gal4, UAS:RFP, sqh-GFP; UAS-bbg^RNAi L3 wing disc stained with anti-Bbg (A) and Sqh-GFP (endogenous signal, A′). (B and B′) Pouch of en-Gal4, UAS:RFP, sqh-GFP L3 wing disc stained with anti-Bbg (B) and Sqh-GFP (endogenous signal, B′), respectively. (C) Ratio of fluorescence intensity of Sqh-GFP in en-Gal4, UAS:RFP, sqh-GFP; UAS-bbg^RNAi and en-Gal4, UAS:RFP, sqh-GFP L3 wing discs (six independent discs per genotype). (D) WB of protein extracts isolated from sqh-GFP and sqh-GFP;bbg^B211 L3 wing discs, showing a reduction of total and phosphorylated Sqh-GFP in sqh-GFP;bbg^B211. Tubulin served as loading control. Antibodies used were anti-Bbg, anti-GFP (for both Sqh-GFP and Phospho-Sqh-GFP), and antitubulin. (E) IP from protein extracts isolated from WT and sqh-GFP L3 wing discs, using an anti-GFP antibody (three biological replicates per condition). Bbg is immunoprecipitated from extracts of Sqh-GFP (two right lanes), but not from WT extracts (two left lanes). The statistical analysis (C) used t test and ANOVA. **, P ≤ 0.01. Error bar shows SD. Bars, 25 µm.