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. 2018 Mar 5;217(3):1047–1062. doi: 10.1083/jcb.201705107

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© 2018 Forest et al.

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Figure 4. — bbgcan modify the overgrowth of spectrin mutants. (A–F) Representative adult female Drosophila wings after nubbin-Gal4 (nubG4)-driven RNAi knockdown of the indicated genotypes. Lowering bbg dosage can modify (reduce) the overgrowth induced by kst or α-Spec RNAi. (G) Quantification of the effects shown in A–F. The wing area for each genotype is expressed as a ratio compared with the mean wing area of the nubG4>UAS GFP controls (Ctrl). SD is shown; unpaired two-tailed Student’s t test on raw data; **, P < 0.01; ***, P < 0.001; n = 17–22 independent female wings. (H–J) Quantification of the mean FRT80B mutant clone total areas (H), cell numbers (I), and apical cell sizes (J) of the indicated genotypes shown as the mean ratio between individual mutant tissue clones and their corresponding WT twin spots as shown in Fig. 2. Controls are “empty” clones performed with FRT80B. SEM is shown; unpaired two-tailed Student’s t test; *, P < 0.05; n = 10 clone pairs from independent discs.