Figure 6.

The upstream Yki negative regulators Crb and Ex are not affected in bbg mutants. (A and B) In bbg mutant clones stained for E-Cad (blue) and marked by the absence of Bbg (A, red) or β-Gal (B, red), the localization and levels of Crb (A, green) or Ex (B, green) were not affected or weakly reduced (compare yellow arrowheads [mutant] with yellow asterisks [WT]). Bars, 10 µm. (C and D) Genetic interaction between bbg and ex. Although overexpressed ex under the control of the nubG4 driver led to strongly reduced wings (C), this effect was further enhanced when bbg was knocked down by RNAi (D). (E and F) Genetic interaction between bbg and hpo. Although the knockdown of hippo by RNAi under the control of the engrailed-Gal4 (enG4) driver led to strongly enlarged wings (E), this effect could not be modified by further impairing bbg (F). (G) Quantification of the effects shown in C–F. The wing area for each genotype was expressed as a ratio compared with the mean wing area of controls (nubG4>UAS GFP controls on the left, and enG4>UAS GFP controls on the right). SD is shown; unpaired two-tailed Student’s t test on raw data; ***, P < 0.001; n = 17–22 independent female wings.