Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Mar 5;217(3):861–876. doi: 10.1083/jcb.201709005

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Petsalaki et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 4. — Chmp4c is required for optimal localization of spindle checkpoint proteins to prometaphase kinetochores. Localization of ZW10 and Rod (A–C), Mad1 and Mad2:GFP (D–F), and BubR1 (G) in cells transfected with negative siRNA (control) or Chmp4c siRNA (siChmp4c). Relative green/red fluorescence intensity is shown, and values in the control were set to one. n > 200 kinetochores, 20 cells from three independent experiments. Error bars show the SD. ***, P < 0.001 compared with control. Student’s t test was used. In some Chmp4c-depleted cells, a few (typically two to six) prometaphase kinetochores exhibited detectable Mad1 or Mad2:GFP staining and are shown in insets with dotted lines (D and E). Insets show 1.7× magnification of kinetochores. Bars, 5 µm.