Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Mar 5;217(3):861–876. doi: 10.1083/jcb.201709005

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Petsalaki et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 8. — Expression of Chmp4c:Mis12:GFP induces accumulation of metaphase cells. (A) Cells were transfected with GFP, WT, or mutant (L228A) Chmp4c fused to Mis12:GFP in the absence or presence of ZW10 siRNA (siZW10) and analyzed for mitotic index and phenotypes at 24 h after transfection. GFP, tubulin, and DNA staining were used to determine mitotic index and fraction of cells in metaphase, anaphase, or all other mitotic states. n = 300 cells per condition from three independent experiments. (B–D) Localization of ZW10 or Mad1 at metaphase plate in cells treated as in A. Relative red fluorescence intensity is shown, and values in WT Chmp4c:Mis12:GFP were set to one. n > 100 kinetochores, 10 cells from three independent experiments. Error bars show the SD. ***, P < 0.001 compared with the control. Statistically significant differences were determined by ANOVA and Student’s t test. Insets in B and C show 1.7× magnification of kinetochores. Bars, 5 µm. (E and F) Model for the role of Chmp4c in the spindle checkpoint. MTs, microtubules; dashed arrow, RZZ binding to Chmp4c. See text for details.