Figure 4.

Curcumin inhibits MCL-1 in HemECs. HemECs were treated with 25 μM curcumin or DMSO for 48 hours. (A) The mRNA level of MCL-1 in treated cells was examined by the RT-PCR assay. (B) The protein level of MCL-1 in treated cells was examined by western blotting analysis. GAPDH was used as a loading control. DMSO = dimethyl sulfoxide, GAPDH = glyceraldehyde-3-phosphate dehydrogenase, HemECs = hemangioma endothelial cells, MCL-1 = myeloid cell leukemia-1, RT-PCR = real-time polymerase chain reaction.