Figure 5.

Curcumin triggers apoptosis signaling in HemECs. (A) HemECs were treated with 25 μM curcumin or DMSO for 48 hours. The protein levels of cl-casp3 and PARP (full length-PARP and cleaved PARP) were examined by western blotting. GAPDH was used as a loading control. (B) HemECs were treated with 25 μM curcumin or DMSO for 48 hours. The level of caspase-3 activity was examined with Caspase-3 Colorimetric Protease Assay Kit according to manufactory protocol. The data are presented by mean ± standard deviation of 3 independent experiments. Cl-casp3 = cl-caspase-3, DMSO = dimethyl sulfoxide, GAPDH = glyceraldehyde-3-phosphate dehydrogenase, HemECs = hemangioma endothelial cells, PARP = poly(adenosine diphosphate ribose) polymerase.