Table 1.
Plasmids used in this study
| Vector | Promoter | Antibiotic markera | Gene/enzyme inserts | Replicon | Source/ Reference |
|---|---|---|---|---|---|
| pENTR™/SD/D-TOPO® | T7 | Kan | None | pUC | Invitrogen |
| pIRS | T7 | Spec | dxs, idi, dxr | CloDF13 | [23] |
| pGG_DEST | T7 | Cam | GGPPS | P15A | [23] |
| pGG_coTS79 | T7 | Cam | GGPPS, TS | P15A | This study |
| pCW′_2A6_hNPR | tac | Amp | CYP2A6, hNPR | M13 | [24] |
| pCW′_coCYP725A4/coTCPR (fused) | tac | Amp | CYP725A4, TCPR | M13 | This study |
| pCW′_coCYP725A4_hNPR (bicistronic) | tac | Amp | CYP725A4, hNPR | M13 | This study |
| pCW′_NcoI_coCYP725A4_hNPR (bicistronic) | tac | Amp | CYP725A4, hNPR | M13 | This study |
| pCW′_coCYP725A4_TCPR (bicistronic) | tac | Amp | CYP725A4, TCPR | M13 | This study |
| pCW′_coCYP725A4_coCYP725A4/coTCPR (bicistronic/fused) | tac | Amp | CYP725A4, CYP725A4, TCPR | M13 | This study |
| pCW′_coTxCb5_coCYP725A4/coTCPR (bicistronic/fused) | tac | Amp | TxCb5, CYP725A4, TCPR | M13 | This study |
a
Kan= kanamycin; Spec = spectinomycin; Cam = chloramphenicol; Amp = ampicillin