Substrate type and cellular contractility influence YAP nuclear localization in MSCs. (A) YAP staining (red), F-actin staining (green), and nuclear staining (blue), scale bar = 20 μm. (B) Quantification of nuclear localization at different passages and with inhibition of contractility (n = ~20, *: p<0.05 vs. TCP using two-way ANOVA across groups and passage number only with Fisher’s LSD, +: p<0.05 vs. P0 TCP from one-way ANOVA across only TCP groups, Fisher’s LSD, Y27: Y27632, Rho kinase inhibitor, mean ± S.E.M.).