Figure 6. Functional conservation of the LXR-MeXis axis in humans.

A. Sequence similarity across species at the MeXis gene locus. B. ABCA1 and TCONS00016111 expression in differentiated THP-1 monocytes treated with DMSO (Ctrl) or GW3965 (0.5 μM) for 16 hours (N=4 per group). Mean ± SD. * P < 0.05 by two-sided student’s t-test. C. ABCA1 and TCONS00016111 expression in differentiated THP-1 cells treated with the indicated ASOs (50 nM) and GW3965 (0.5 μM) (N=3 per group). Data are mean ± SD. * P < 0.05; ** P < 0.01; *** P < 0.001 by one-way ANOVA followed by multiple comparisons test (Dunnett’s).D. Cholesterol efflux in the presence of ApoA-I of THP-1 cells treated with the indicated ASO, loaded with [³H]cholesterol (1.0 μCi/ml), and treated with acyl-CoA:cholesterol O-acyltransferase inhibitor (2 μg/ml) and with either DMSO or LXR ligand (1 μM GW3965) (n=3/group). Data are mean ± SD. * P < 0.05 by two-sided student’s t-test. E. Abca1 gene expression in MeXis^−/− BMDM macrophages treated with control (antisense MeXis) or MeXis lentivirus (n=3 per group). Data are mean ± SD. * P < 0.05 by two-sided student’s t-test. F. Abca1 expression in THP-1 cells treated with control or MeXis lentivirus (n=3 per group).Mean ± Two-sided student’s t-test. Not significant at P<0.05. 95% Confidence Interval (-0.1025 to 1.338). G. Regional association plot of variants at TCONS-0016111 and the risk of coronary artery disease in humans from the CARDIoGRAMplusC4D Consortium.