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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: Hum Mutat. 2018 Jan 19;39(4):550–562. doi: 10.1002/humu.23397

FIGURE 3. CYP26B1 NTD-variants attenuate function as assessed by modulation of RA-mediated gene transcription.

FIGURE 3

A–H) Real-time quantitative PCR assays were performed to test the mRNA levels of genes that are downstream of RA signaling and involved in neural development. NE-4C cells were transfected with CYP26B1 WT or mutant plasmids and treated for 24 hours with RA. Gapdh gene was used as a loading control. The level of expression is indicated as fold change relative to WT vector, which was set to 1. All experiments were from three biological replicates. ***: P < 0.0001; **: P < 0.01; *: P < 0.05; Student’s t-test.