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. Author manuscript; available in PMC: 2019 Mar 15.
Published in final edited form as: J Immunol. 2018 Feb 9;200(6):1982–1987. doi: 10.4049/jimmunol.1701676

Figure 2. CD45.1 strain bear a point mutation in the Ncr1 locus causing loss of NCR1 expression.

Figure 2

NCR1 expression on (A) spleen NK cells (CD3−NK1.1+), (B) NK cells from the indicated organs, and (C) intestinal ILCs. (D) Surface and intracellular expression of NCR1 using monoclonal (mAb) or polyclonal (pAb) antibodies. (E) NCR1 expression on NK cells from CD45.1 recipients 3 weeks after gut content transfer. (F) NCR1 expression on donor-derived NK cells from CD45.2+ or CD45.1+ cells. (G) Genomic DNA and mRNA transcripts of Ncr1 gene. (H) mRNA and protein sequence of Ncr1 gene. (I) Amounts of NCR1 proteins from sorted NK cells. (J, K) NCR1 expression in mutant (NCR1C14R) and wild-type (NCR1WT) transfected cells. Data are representative of three independent experiments with n = 4 per group. Statistics were analyzed using t-test, ****p < 0.0001.